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ABI Applied Biosystems 7300 Real-Time PCR System

$5,500.00

THE BIOSYSTEMS ABI 7300 REAL-TIME PCR SYSTEM IS AN AFFORDABLE SOLUTION FOR BUDGET-CONSCIOUS LABS. THE FOUR COLOR DETECTION SYSTEM GIVES USERS THE FLEXIBILITY TO RUN A VARIETY OF APPLICATIONS INCLUDING SNP GENOTYPING, GENE EXPRESSION, PLUS/MINUS ASSAYS, PATHOGEN QUANTITATION AND MORE. THE INSTRUMENT IS EQUIPPED WITH A CCD CAMERA FOR PRECISION OPTICS AND COMES WITH ADVANCED MULTI-COMPONENTING ALGORITHMS FOR ACCURATE AND REPRODUCIBLE RESULTS.
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Description

ABI Applied Biosystems 7300 Real-Time PCR System

The recent advances in PCR and especially the development of quantitative real-time PCR (qPCR, also known as real-time PCR) make that methodology a staple of almost any biological laboratory, with myriads of applications in almost any bioscience discipline. In qPCR, DNA amplification is monitored in real time using fluorescent dyes. This improvement provides more accurate and sensitive measurements of e.g. gene expression, pathogen detection, and allelic discrimination, than traditional (end-point) PCR. To achieve high quality results of qPCR, a superb apparatus is needed and this is where you can turn to the Applied Biosystems 7300 Real-Time PCR System.

The Biosystems ABI 7300 Real-Time PCR System is a very reliable platform with a high quality Peltier-based heating block providing uniform heating (and cooling) of all the wells. It works equally well with both 96-well plates and 0.2 ml tubes, except you must have the converter to utilize both the 96-well format and 0.2 ml tubes. I use the tubes and plates from Applied Biosystems although I have found that Fisher sells the equivalent for cheaper (I haven’t tested them yet). Convenient features include the real-time monitoring of amplification during the run. (Although I am not one to sit and watch the entire run!) I have been using this instrument to detect expression of many protein-encoding genes and to determine drug-induced changes in the expression of these genes.

The Biosystems ABI 7300 Real-Time PCR System uses four-color detection providing the flexibility to perform a variety of applications including gene expression analysis, pathogen
quantitation, SNP genotyping, and isothermal. The versatile software has plate setup wizards which guide you through experimental setup and include advanced data viewing capabilities. Thanks to the precision optics and a chargecoupled device (CCD) camera with a sophisticated multicomponenting algorithm you get highly accurate, reproducible, and reliable results. Features include: sample wells illuminated with tungsten-halogen lamps; and powerful and user-friendly, sequence detection software.

Biosystems ABI 7300 Real-Time PCR System Specifications:ABI Applied Biosystems 7300 Real-Time PCR System

– Thermal cycling system: Peltier-based, 96-well block
– Sample Ramp Rate: +/-1.1°C/sec
– Peak Block Ramp Rate: 1.6°C/sec
– Temperature Range: 4°C – 100°C
– Temperature Accuracy: ±0.25°C of setpoint/display temperature measured  at 3 minutes after clock start
– Temperature Uniformity: ±0.50°C, 30 seconds after clock start
– Optical system : Single excitation, four emission filters, and CCD camera
– Passive Reference Dyes: ROX  or any calibrated dye.
– Data Collection: Data collected in all 4 filters for all wells regardless of plate setup. Plate setup may be altered after run completes.
– Quantitative PCR run time: < 1 hour 50 minutes
– Supported Volumes: 20 – 100 μL

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SettingsABI Applied Biosystems 7300 Real-Time PCR System removeLutronic Clarity II For Sale removeConvergent Solea 2.0 Dental Diode Laser For Sale removeENDYMED PURE PLUS FOR SALE removeALMA SOPRANO XLI WITH SHR DIODE & NIR FOR SALE removeCUTERA SECRET RF 2.0 FOR SALE remove
NameABI Applied Biosystems 7300 Real-Time PCR System removeLutronic Clarity II For Sale removeConvergent Solea 2.0 Dental Diode Laser For Sale removeENDYMED PURE PLUS FOR SALE removeALMA SOPRANO XLI WITH SHR DIODE & NIR FOR SALE removeCUTERA SECRET RF 2.0 FOR SALE remove
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Description
THE BIOSYSTEMS ABI 7300 REAL-TIME PCR SYSTEM IS AN AFFORDABLE SOLUTION FOR BUDGET-CONSCIOUS LABS. THE FOUR COLOR DETECTION SYSTEM GIVES USERS THE FLEXIBILITY TO RUN A VARIETY OF APPLICATIONS INCLUDING SNP GENOTYPING, GENE EXPRESSION, PLUS/MINUS ASSAYS, PATHOGEN QUANTITATION AND MORE. THE INSTRUMENT IS EQUIPPED WITH A CCD CAMERA FOR PRECISION OPTICS AND COMES WITH ADVANCED MULTI-COMPONENTING ALGORITHMS FOR ACCURATE AND REPRODUCIBLE RESULTS.

Advanced ergonomic handpiece with multi-spot, quick-zoom cartridges, and large touch screen GUI.

Convergent Solea 2.0 Dental Diode Laser Specifications Manufacturer: Convergent Model: Solea 2.0 Power Requirements: Not available Year: 2015 Laser Style: Cart Fiber-Optic Trunk Condition:Functional Laser Type:All-Tissue Software Version: 3.2.2The PURE+ is offered in two configurations:FSR + TC: Fixed FSR handpiece + one exchangeable connector suitable for all TC handpieces: iFine, Small and Large TC Complete: Two exchangeable connectors on each side, suitable for all TC handpieces: iFine, Shaper, Small and LargeThe iFine treatment Handpiece – specially engineered to target fine lines and wrinkles found in the delicate thin – skin areas around the eyes, providing: Smoother, tighter skin around the eyes. Reduced under eye sagging and improved eye contour. Reduced hooding to reveal more of the eyelid. More youthful, less tired looking eyes. Softening of fine line enabling easier application of make-up.
Alma Soprano XLi with SHR Diode & NIR Handpieces;  Excellent Cosmetic and Operating Condition, Single Owner, Full Maintenance Service, Calibration, and Replacement of Flow Switch Performed on 08/30/2019, Record Available Upon Request; Original SHR Total Pulse Count = 10,241,483; NIR Total Pulse Count =2,545,712 ;
Includes:
(1) 810 Diode Handpiece with Sapphire Dual Chill Tip and 12×10 mm Spot Size,
(1) NIR Skin Tightening Handpiece,
(2) Keys,
(1) Footswitch,
(1) Operator Manual,
(1) Consult Book,
(1) Water Refill Kit,
(1) Warning Sign, and 90 Day Warranty.km
The ability to work specifically in the deep dermis, without damaging the epidermis, is regulated in the RF Secret by the delay time of fractional radiofrequency. The needles are inserted at a rate of milliseconds equal to a stamping process in the skin and also withdrawn immediately. Only after the needles are penetrated into the desired skin layer, is carried out for a few milliseconds, a radio frequency pulse to set heat damage. While the penetration into the skin, the needles remain always cold. In order to achieve a deep and superficial effect, the needle depths can be varied individually in the respective treatment passes.
Content

ABI Applied Biosystems 7300 Real-Time PCR System

The recent advances in PCR and especially the development of quantitative real-time PCR (qPCR, also known as real-time PCR) make that methodology a staple of almost any biological laboratory, with myriads of applications in almost any bioscience discipline. In qPCR, DNA amplification is monitored in real time using fluorescent dyes. This improvement provides more accurate and sensitive measurements of e.g. gene expression, pathogen detection, and allelic discrimination, than traditional (end-point) PCR. To achieve high quality results of qPCR, a superb apparatus is needed and this is where you can turn to the Applied Biosystems 7300 Real-Time PCR System.The Biosystems ABI 7300 Real-Time PCR System is a very reliable platform with a high quality Peltier-based heating block providing uniform heating (and cooling) of all the wells. It works equally well with both 96-well plates and 0.2 ml tubes, except you must have the converter to utilize both the 96-well format and 0.2 ml tubes. I use the tubes and plates from Applied Biosystems although I have found that Fisher sells the equivalent for cheaper (I haven’t tested them yet). Convenient features include the real-time monitoring of amplification during the run. (Although I am not one to sit and watch the entire run!) I have been using this instrument to detect expression of many protein-encoding genes and to determine drug-induced changes in the expression of these genes.
The Biosystems ABI 7300 Real-Time PCR System uses four-color detection providing the flexibility to perform a variety of applications including gene expression analysis, pathogen quantitation, SNP genotyping, and isothermal. The versatile software has plate setup wizards which guide you through experimental setup and include advanced data viewing capabilities. Thanks to the precision optics and a chargecoupled device (CCD) camera with a sophisticated multicomponenting algorithm you get highly accurate, reproducible, and reliable results. Features include: sample wells illuminated with tungsten-halogen lamps; and powerful and user-friendly, sequence detection software.Biosystems ABI 7300 Real-Time PCR System Specifications:ABI Applied Biosystems 7300 Real-Time PCR System– Thermal cycling system: Peltier-based, 96-well block – Sample Ramp Rate: +/-1.1°C/sec – Peak Block Ramp Rate: 1.6°C/sec – Temperature Range: 4°C – 100°C – Temperature Accuracy: ±0.25°C of setpoint/display temperature measured  at 3 minutes after clock start – Temperature Uniformity: ±0.50°C, 30 seconds after clock start – Optical system : Single excitation, four emission filters, and CCD camera – Passive Reference Dyes: ROX  or any calibrated dye. – Data Collection: Data collected in all 4 filters for all wells regardless of plate setup. Plate setup may be altered after run completes. – Quantitative PCR run time: < 1 hour 50 minutes – Supported Volumes: 20 – 100 μL

Lutronic Clarity II

Lutronic’s Clarity II is a high-powered, dual-wavelength platform that effectively delivers a wider range of parameters to help safely and efficiently treat the broadest indications with ease. The innovative design includes: a single fiber for all spot sizes(2-25mm), intuitive interface, and no need for calibration after any parameter change. Clarity II can save time and help facilitate faster, more effective treatments. Higher power 755/1064nm platform for effective treatments up to 45%* faster. Brilliantly removes hair, pigment, and vascular lesions in all skin types. IntelliTrak technology and Temperature Sensing enable increased provider confidence and treatment efficacy.Clarity II Key Feature 1. Powerful and faster Provide up to 25mm spot size at a useable >2 Hz High power performance Up to 45% faster 2. Intelligent and convenientIntelliTraking system to prevent skip area and consistent treatment Real time skin temperature monitoring system 3. Designed for versatilityIntelligent memory function to keep track of past treatment history in detail Zoom cartridge system for treatment areaLutronic Clarity IIHow It Works The CLARITY II Laser System contains two separate laser resonators, Alexandrite and an Nd:YAG, which generate pulse laser energy at the nominal wavelength of 755nm and 1064nm respectively. The output of each laser generator and the 515nm aiming beam are optically combined on the laser rail so that their beam paths are identical as they exit the laser system. This allows the use of a single delivery system for either the 755nm or 1064nm wavelength.Clarity II with IntelliTrak technologyHigher-powered 755/1064nm platform laser Up to 2X faster Laser Hair Removal Txs* Exclusive shorter pulsewidths available including 1ms setting for finer facial hairs Breakthrough intelligent capabilities IntelliTrak technology for faster more consistent Tx coverage Temperature Sensing for real-time skin temp feedback during Tx Auto-calibration in seconds saves time and improves productivity Advanced, easy-to-use platform Single fiber for all 2-24mm spot cartridges Numerous skin rejuvenation capabilities including vascular, pigment, and toning Txs *At settings of: 755nm, 5ms, 12J/cm2: Clarity II at 22mm, 3Hz, IntelliTrak at 18% overlap vs. older system at 18mm, 2Hz, and est. ~18% manual overlap. Geometric treatment coverage speed is 11.4cm/second with Clarity II vs. 5.1cm/second with older system. 

Convergent Solea 2.0 Dental Diode Laser

OVERVIEWMakers of the Solea All-Tissue LaserConvergent Dental, Inc. is a privately owned dental equipment and technology company. The Company’s flagship product, Solea, is a breakthrough technology that enables virtually every cavity prep to be performed anesthesia-free, delivering a dental experience that patients prefer. Solea also enables soft tissue procedures that are blood-free and suture-free with minimal post-op discomfort and remarkably rapid healing.Solea offers a completely different experience for both dentists and patients compared to traditional instruments or earlier laser technology. Solea dramatically increases new patient flow, enables better clinical outcomes, makes you more efficient, and allows you to perform procedures previously referred out.TECHNOLOGY Convergent Solea 2.0 Dental Diode LaserUnique 9.3 µm CO2 LaserOur unique 9.3 wavelength is highly absorbed in tooth structure. The result? Solea actually vaporizes enamel (unlike other lasers that slowly chip it away), giving you the power to work anywhere in the oral cavity from any angle with speed and ease. Whether treating virgin teeth, replacing old restorations, or performing soft or osseous tissue procedures, Solea is the right tool for the job.WHY SOLEAPatients prefer fillings without a needle or a drill.Solea is a breakthrough technology that enables virtually every cavity prep to be performed anesthesia-free, delivering a dental experience patients prefer. Solea also enables soft tissue procedures that are blood-free and suture-free with minimal post-op discomfort and remarkably rapid healing.
An Experience That Moves Patients
By eliminating the primary causes of patient anxiety (the needle and the drill), Solea delivers a dental experience patients prefer. When needles, numbness, and the sound of the drill, and the pain are all removed from a patient’s dental experience, two important things happen: 1) Patients never settle for a traditional experience again, and 2) They encourage their family and friends to experience the same.
Deliver Better Outcomes
Enjoy the highest degree of control over procedures and practice truly minimally invasive dentistry. For hard tissue, Solea allows for selective tissue ablation, which results in the preservation of healthy tooth structure and minimized risk of iatrogenic damage. Solea also provides a bactericidal effect because laser energy kills all bacteria with which it comes in contact. For soft and osseous tissue, Solea enables precise control and is both atraumatic and virtually blood-free, allowing you to deliver better outcomes than you could deliver using traditional instruments.
Work Smarter, Not Harder
By eliminating anesthesia, Solea removes obstacles that slow you down and limit what you can accomplish in a single visit. No more calming patients down, injecting the anesthesia, waiting for it to take effect, and scheduling multiple visits for multi-quadrant dentistry. With Solea, you simply greet the patient, get started, and stay until treatment is complete. Enjoy more control over your schedule and feel like you are working less.
Expand Your Practice
Solea dentists perform soft and osseous tissue procedures because they are simple, fast, and minimally invasive. By virtually eliminating bleeding, sutures, and post-operative pain, you can add higher revenue procedures – such as gingivectomies, frenectomies, and flapless crown lengthening – previously referred out or ignored. Your patients will love receiving treatment from a dentist they know and trust, and they will be amazed by the remarkably rapid healing process and absence of post-operative pain.
ENDYMED PURE PLUS Input Power:100-120V, 50-60Hz, 2A or 220-240V, 50-60Hz, 1A Power Consumption:220 W Fuses:2 x 1.6A for 220V; 2 x 3.15A for 110V Output Power:Up to 50 Watts Output Frequency:1MHz Working Temperature:+10°C – +25°C Dimensions:(H x W x D) 600 x 305 x 365 mm Weight:10 kg GOOD THINGS COMES IN SMALL PACKAGES – THE PURE+ The Pure+ by Endymed Pure Plus, powered by our FDA cleared, patented 3DEEPRF technology. The PURE+ offers professional grade Fractional Skin Resurfacing and superior body, facial and neck tightening and contouring treatments, all on one easy to use, compact platform.The PURE+ offers complete flexibility with exchangeable treatment handpieces – one PURE+ console can be configured any way you like to provide you and your clients with the most effective and popular treatments available today.The PURE+ is offered in two configurations:FSR + TC: Fixed FSR handpiece + one exchangeable connector suitable for all TC handpieces: iFine, Small and Large TC Complete: Two exchangeable connectors on each side, suitable for all TC handpieces: iFine, Shaper, Small and LargeThe iFine treatment Handpiece – specially engineered to target fine lines and wrinkles found in the delicate thin – skin areas around the eyes, providing: Smoother, tighter skin around the eyes. Reduced under eye sagging and improved eye contour. Reduced hooding to reveal more of the eyelid. More youthful, less tired looking eyes. Softening of fine line enabling easier application of make-up.3Deep Small-Medium Handpiece Designed to treat wrinkles and lax skin on the face, neck and upper body.3Deep Fractional Skin Resurfacing Handpiece Specially designed for fractional skin resurfacing and treatment of skin roughness, hyperpigmentation, and wrinkles and scars on both facial and body. The FSR handpiece contains a matrix of 112 tiny electrodes, allowing for the first time simultaneous fractional micro-ablation of the epidermis, together with volumetric heating of 100% of the dermis. This technology provides the capability to differentiate between micro-ablation and dermal heating which appears to be the optimal multi-layer treatment to affect aging skin and atrophic acne scars.3Deep Shaper Handpiece Major innovation in RF skin tightening, body shaping and contouring, and cellulite treatment for large body areas. The handpiece’s unique engineered electrode surface is 40.7 cm2 allowing for shorter treatment times (20 minute abdomen treatment, 30 minute cellulite treatments for the thighs and buttocks). Deeper, controlled energy penetration tightens and firms the skin by stimulating the body’s natural collagen renewal process, providing better and more effective results.

ALMA SOPRANO XLI WITH SHR DIODE & NIR

2012 Alma Soprano XLi with SHR Diode & NIR Handpieces; Manufactured 10/2012; Excellent Cosmetic and Operating Condition, Single Owner, Full Maintenance Service, Calibration, and Replacement of Flow Switch Performed on 08/30/2017, Record Available Upon Request; Original SHR Total Pulse Count = 10,241,483; NIR Total Pulse Count =2,545,712 ;
Includes:
(1) 810 Diode Handpiece with Sapphire Dual Chill Tip and 12×10 mm Spot Size,
(1) NIR Skin Tightening Handpiece,
(2) Keys,
(1) Footswitch,
(1) Operator Manual,
(1) Consult Book,
(1) Water Refill Kit,
(1) Warning Sign, and 90 Day Warranty.km
Product Specifications
Light SourceDiode (continuous wave)
Wavelength810 nm
FluenceUp to 120 J/cm2
Pulse Duration10 – 1,350 ms
Spot Size12 x 10 mm
Repetition RateUp to 10 Hz SHR Mode, 3 Hz Pulsed Mode
CoolingDualChill contact cooling, plus integrated adapter for Zimmer Cryo 5
Electrical120/230 VAC, 20/10 A, 50/60 Hz
Dimensions20″ x 22.4″ x 47″
Weight110 lbs
 
Manufacturer Notes
Virtually painless hair removal

Like earlier generations of the Soprano Family, The Soprano XL is the perfect solution to differentiate your practice in today’s cluttered laser hair removal market. Revolutionary pain-free Soprano treatments have redefined laser hair removal using the gold standard 810-nm diode and Alma’s IN-Motion technology. The Soprano’s virtually pain-free hair removal offers:

– Gold standard 810-nm laser diode provides reliable results
– Sapphire DualChill tip and optional Zimmer Cryo 5 adapter
– Permanent hair reduction of all pigmented hair on all skin types, including tanned skin
– IN-Motion technology for virtually pain-free hair removal
– Faster treatment times – huge 12×10 mm spot, up to 10 Hz repetition rate
– Low cost of ownership with no disposables required
– Easy to operate

The Soprano Family of platforms are the ideal standalone hair removal system that is safe, reliable, easy to use – and virtually painless. Through patient-friendly Pain-Free, Hair-Free hair removal procedures, the Soprano adds a proven revenue stream to your practice quickly and easily, as well as deep heating treatments through its NIR handpiece. Alma Lasers supports your Soprano investment through the Alma Lasers Marketing Assistance (A.L.M.A.) program, Office by Alma marketing materials, and an ongoing schedule of training programs.

Near-Infrared (NIR) module
Dermal heating through uniform energy distribution, effective for treating minor muscle pain, joint stiffness and minor muscle strains. The Soprano’s NIR offers:
Fast dermal heating – huge 18 cm2 spot
Easy to use
CUTERA SECRET RF 2.0CUTERA SECRET RF 2.0 MICRONEEDLING RADIO is a fractional micro-needling system (fractional radiofrequency) that exceeds the capabilities and limitations of fractional laser significantly. It is a system for extensive skin structure improvement and treatment of hyperhidrosis (radio frequency thermotherapy RFTT), based on both monopolar and fractionated on, bipolar radio frequency. An exact adjustment of the depth of the microneedles and the “cold” passing the epidermis allow the energy output directly into the target layer. The needle depth is 0.5 mm to 3.5 mm can be varied, thereby opening up a wide range of treatments. The adjustable intensity of the RF energy allows for the treatment of various areas of the body with different energy performance.The ability to work specifically in the deep dermis, without damaging the epidermis, is regulated in the RF Secret by the delay time of fractional radiofrequency. The needles are inserted at a rate of milliseconds equal to a stamping process in the skin and also withdrawn immediately. Only after the needles are penetrated into the desired skin layer, is carried out for a few milliseconds, a radio frequency pulse to set heat damage. While the penetration into the skin, the needles remain always cold. In order to achieve a deep and superficial effect, the needle depths can be varied individually in the respective treatment passes.Dermal Remodeling from the Inside Out Demand for non-surgical aesthetic procedures with little to no downtime is on the rise. More than 70% of patients are bothered by signs of aging such as fine lines and wrinkles as well as skin texture and discoloration.1Secret RF is a novel fractional radio frequency (RF) micro-needling system for tissue coagulation and hemostasis designed to stimulate and remodel collagen and address the common signs of aging. It is ideally suited for use on patients who wish to revitalize and refresh the appearance of their skin on all skin types. By adjusting the micro-needles, Secret RF can deliver energy at various depths, so that treatments can be tailored to address each patients individual concerns such as fine lines, wrinkles, acne scars, photoaging and striae at the depth required.Secret RF System Secret RFs unique technology and customized delivery of energy enables providers to tailor treatments for a variety of skin concerns on all skin types.Customized Dermal Remodeling Secret RF induces dermal remodeling by applying precisely controlled RF energy directly into various depths of skin  from 0.5-3.5 mm – with minimally invasive micro-needles to spare the epidermis and reduce patient downtime.

Skin Revitalization and Resurfacing

Secret RF is a radio frequency (RF) fractional microneedling system that helps you deliver tailored energy to improve fine lines, wrinkles, and scars from the inside out.

Secret RF in action

Applications include fine lines and wrinkles, photoaging, scarring, acne scars, striaeSecret RF technology

Handpieces

Two sizes of treatment tips – 25 and 64 pin – give you customizable options for treating the face and body with precision and efficiency.
Power:20 W ± 20% RF frequency:2 Mh ± 20% Intensity:0 ~ 100 Level (10 levels) Repeat:0.2 / 0.5 / 1/2 sec / individually. Needle Size:0.3 mm Info about needles:25 electric needles (5 x 5, 7.8 mm x 7.8 mm / height: 1.95 mm (standard) and 16 electric needles (4 x 4, 7.8 mm x 7.8 mm / height 2.6 mm (optional) Treatment range:1 x 1cm Adjustable Depth:0.5 ~5 mm (0.1 steps) Treatment duration:50 ms ~ 950 ms Electrical power:100 ~ 240 VAC, 50/60 Hz, 220 VAMonopolar RF Power:520 W ± 20% RF frequency:2 Mh ± 20% RF electrode:monopolar Repeat:CW RF electrode size:15APPLICATIONSFacialsNon-surgical facelift Skin tightening Pore reduction Wrinkle reduction Skin Rejuvenation (illumination) Acne scars Body treatmentsScar Keratosis pilaris (Keratosis pilaris) Stretch marks
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